Search of human proteins with 189011548

Search of human proteins with 189011548

BLASTP 2.2.11 [Jun-05-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|189011548 N-acylsphingosine amidohydrolase 1 isoform a
preproprotein [Homo sapiens]
         (395 letters)

Database: hs.faa 
           37,866 sequences; 18,247,518 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|189011548 N-acylsphingosine amidohydrolase 1 isoform a prepro...   820   0.0  
gi|189011546 N-acylsphingosine amidohydrolase 1 isoform b [Homo ...   769   0.0  
gi|189011550 N-acylsphingosine amidohydrolase 1 isoform c [Homo ...   693   0.0  
gi|109148550 N-acylethanolamine acid amidase isoform 1 precursor...   191   1e-48
gi|109148548 N-acylethanolamine acid amidase isoform 2 precursor...   170   2e-42
gi|134031945 SCO-spondin [Homo sapiens]                                33   0.34 
gi|4506203 proteasome beta 7 subunit proprotein [Homo sapiens]         29   8.5  

>gi|189011548 N-acylsphingosine amidohydrolase 1 isoform a
           preproprotein [Homo sapiens]
          Length = 395

 Score =  820 bits (2118), Expect = 0.0
 Identities = 395/395 (100%), Positives = 395/395 (100%)

Query: 1   MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYK 60
           MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYK
Sbjct: 1   MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYK 60

Query: 61  RWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIA 120
           RWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIA
Sbjct: 61  RWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIA 120

Query: 121 AVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITE 180
           AVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITE
Sbjct: 121 AVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITE 180

Query: 181 QLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWI 240
           QLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWI
Sbjct: 181 QLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWI 240

Query: 241 LGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKE 300
           LGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKE
Sbjct: 241 LGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKE 300

Query: 301 SLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLST 360
           SLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLST
Sbjct: 301 SLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLST 360

Query: 361 KPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 395
           KPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW
Sbjct: 361 KPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 395


>gi|189011546 N-acylsphingosine amidohydrolase 1 isoform b [Homo
           sapiens]
          Length = 411

 Score =  769 bits (1986), Expect = 0.0
 Identities = 369/369 (100%), Positives = 369/369 (100%)

Query: 27  WTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINT 86
           WTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINT
Sbjct: 43  WTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINT 102

Query: 87  FVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSI 146
           FVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSI
Sbjct: 103 FVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSI 162

Query: 147 VAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAG 206
           VAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAG
Sbjct: 163 VAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAG 222

Query: 207 YVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEA 266
           YVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEA
Sbjct: 223 YVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEA 282

Query: 267 KNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKH 326
           KNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKH
Sbjct: 283 KNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKH 342

Query: 327 PFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLR 386
           PFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLR
Sbjct: 343 PFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLR 402

Query: 387 DCPDPCIGW 395
           DCPDPCIGW
Sbjct: 403 DCPDPCIGW 411


>gi|189011550 N-acylsphingosine amidohydrolase 1 isoform c [Homo
           sapiens]
          Length = 389

 Score =  693 bits (1788), Expect = 0.0
 Identities = 340/376 (90%), Positives = 340/376 (90%), Gaps = 36/376 (9%)

Query: 27  WTEDCRKSTYPPSGPT-------YRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNS 79
           WTEDCRKSTYPPSGPT       YRGAVPWYTINLDLPPYKRWHELMLDKAPV       
Sbjct: 43  WTEDCRKSTYPPSGPTVFPAVIRYRGAVPWYTINLDLPPYKRWHELMLDKAPV------- 95

Query: 80  LKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYEL 139
                                 PGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYEL
Sbjct: 96  ----------------------PGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYEL 133

Query: 140 FTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVF 199
           FTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVF
Sbjct: 134 FTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVF 193

Query: 200 KASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLEN 259
           KASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLEN
Sbjct: 194 KASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLEN 253

Query: 260 STSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQT 319
           STSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQT
Sbjct: 254 STSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQT 313

Query: 320 NYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKG 379
           NYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKG
Sbjct: 314 NYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKG 373

Query: 380 QFETYLRDCPDPCIGW 395
           QFETYLRDCPDPCIGW
Sbjct: 374 QFETYLRDCPDPCIGW 389


>gi|109148550 N-acylethanolamine acid amidase isoform 1 precursor
           [Homo sapiens]
          Length = 359

 Score =  191 bits (484), Expect = 1e-48
 Identities = 116/352 (32%), Positives = 186/352 (52%), Gaps = 33/352 (9%)

Query: 46  AVPWYTINLDLPPYKRW------HELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDE 99
           A P + ++LD  P  RW      ++L L +A + +VI + +   ++  +  GK++  ++ 
Sbjct: 32  AAPRFNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLI--GKVVLELER 89

Query: 100 KLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGR 159
            LP        PF  E++G+    ++ L + +  N+ YE    CTSIVA+D +GH+ HGR
Sbjct: 90  FLPQ-------PFTGEIRGMCDFMNLSLADCLLVNLAYESSVFCTSIVAQDSRGHIYHGR 142

Query: 160 NMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLF 219
           N+D+                  L+ LTV++ F +N +  F  ++F GYVG+ TG  P  F
Sbjct: 143 NLDYP-------------FGNVLRKLTVDVQFLKNGQIAFTGTTFIGYVGLWTGQSPHKF 189

Query: 220 SLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPA 279
           +++ +ER    G +       L ++ +  + +L R  L  S ++E A   L KT ++A  
Sbjct: 190 TVSGDERDK--GWWWENAIAALFRRHIP-VSWLIRATLSESENFEAAVGKLAKTPLIADV 246

Query: 280 YFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKM 339
           Y+I+GG    EG VITR+R    D++ LD   G W+ V+TNYD WK     DDRRT A  
Sbjct: 247 YYIVGGTSPREGVVITRNRDGPADIWPLDPLNGAWFRVETNYDHWKPAPKEDDRRTSAIK 306

Query: 340 CLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDP 391
            LN T Q N+S E ++ +LS  PV N  T+YTT+  ++ G  + Y+    +P
Sbjct: 307 ALNATGQANLSLEALFQILSVVPVYNNFTIYTTV--MSAGSPDKYMTRIRNP 356


>gi|109148548 N-acylethanolamine acid amidase isoform 2 precursor
           [Homo sapiens]
          Length = 323

 Score =  170 bits (431), Expect = 2e-42
 Identities = 104/316 (32%), Positives = 166/316 (52%), Gaps = 31/316 (9%)

Query: 46  AVPWYTINLDLPPYKRW------HELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDE 99
           A P + ++LD  P  RW      ++L L +A + +VI + +   ++  +  GK++  ++ 
Sbjct: 32  AAPRFNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLI--GKVVLELER 89

Query: 100 KLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGR 159
            LP        PF  E++G+    ++ L + +  N+ YE    CTSIVA+D +GH+ HGR
Sbjct: 90  FLPQ-------PFTGEIRGMCDFMNLSLADCLLVNLAYESSVFCTSIVAQDSRGHIYHGR 142

Query: 160 NMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLF 219
           N+D+                  L+ LTV++ F +N +  F  ++F GYVG+ TG  P  F
Sbjct: 143 NLDYP-------------FGNVLRKLTVDVQFLKNGQIAFTGTTFIGYVGLWTGQSPHKF 189

Query: 220 SLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPA 279
           +++ +ER    G +       L ++ +  + +L R  L  S ++E A   L KT ++A  
Sbjct: 190 TVSGDERDK--GWWWENAIAALFRRHIP-VSWLIRATLSESENFEAAVGKLAKTPLIADV 246

Query: 280 YFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKM 339
           Y+I+GG    EG VITR+R    D++ LD   G W+ V+TNYD WK     DDRRT A  
Sbjct: 247 YYIVGGTSPREGVVITRNRDGPADIWPLDPLNGAWFRVETNYDHWKPAPKEDDRRTSAIK 306

Query: 340 CLNRTSQENISFETMY 355
            LN T Q N+S E ++
Sbjct: 307 ALNATGQANLSLEALF 322


>gi|134031945 SCO-spondin [Homo sapiens]
          Length = 5147

 Score = 33.5 bits (75), Expect = 0.34
 Identities = 17/47 (36%), Positives = 19/47 (40%)

Query: 2   PGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVP 48
           PG  C+  VL A A  CA    +PPW          P G  YR   P
Sbjct: 792 PGSDCLCPVLSAYARRCAQEGASPPWRNQTLCPVMCPGGQEYRECAP 838


>gi|4506203 proteasome beta 7 subunit proprotein [Homo sapiens]
          Length = 277

 Score = 28.9 bits (63), Expect = 8.5
 Identities = 17/49 (34%), Positives = 30/49 (61%), Gaps = 1/49 (2%)

Query: 264 EEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQG 312
           EEAKNL+++  I A  +  LG   + + CVI++++ + L  Y +  K+G
Sbjct: 192 EEAKNLVSEA-IAAGIFNDLGSGSNIDLCVISKNKLDFLRPYTVPNKKG 239


  Database: hs.faa
    Posted date:  Aug 4, 2009  4:42 PM
  Number of letters in database: 18,247,518
  Number of sequences in database:  37,866
  
Lambda     K      H
   0.321    0.139    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,702,522
Number of Sequences: 37866
Number of extensions: 699528
Number of successful extensions: 1388
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1376
Number of HSP's gapped (non-prelim): 7
length of query: 395
length of database: 18,247,518
effective HSP length: 104
effective length of query: 291
effective length of database: 14,309,454
effective search space: 4164051114
effective search space used: 4164051114
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.